Cryopreservation of Iberian red deer (Cervus elaphus hispanicus) spermatozoa obtained by electroejaculation

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Cryopreservation of Iberian red deer (Cervus elaphus hispanicus) spermatozoa obtained by electroejaculation

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Title: Cryopreservation of Iberian red deer (Cervus elaphus hispanicus) spermatozoa obtained by electroejaculation
Author: Martínez Pastor, Felipe;Martínez, Félix;Álvarez García, Mercedes;Maroto Morales, Alejandro;García Álvarez, Olga;Soler, Ana J.;Garde López-Brea, Julián;Paz Cabello, Paulino de;Anel Rodríguez, Luis
xmlui.dri2xhtml.METS-1.0.item-contributor: Facultad de Ciencias Biologicas y Ambientales
xmlui.dri2xhtml.METS-1.0.item-area: Biologia Celular
Abstract: We tested extenders and freezing protocols for Iberian red deer semen. Samples were obtained by electroejaculation (10 stags), and analyzed for motility (CASA), viability (propidium ioide), acrosomal (PNA-FITC) and mitochondrial status (JC-1). Samples were diluted in extender, cooled and adjusted for glycerol (extender with higher glycerol concentration), brought to mL−1 and frozen. Four experiments were carried out, repeating sperm analysis after thawing to compare treatments. In a first experiment, seven samples were frozen using Triladyl® (20% egg yolk) and UL extender (Tes–Tris–fructose, 15% egg yolk, 4% glycerol). Triladyl® yielded higher motility after thawing. In a second trial, 17 samples were frozen using Triladyl®, Andromed®, Bioxcell®, and UL with 8% LDL (low-density lipoproteins). Triladyl® and Andromed® performed better than Bioxcell® on motility, and than UL-LDL on viability and acrosomal status. In a third experiment, the performance of freezing the sperm-rich ejaculate fraction versus the whole ejaculate was tested on nine samples. The sperm-rich ejaculate fraction not only rendered more motile and viable spermatozoa but also showed higher freezability (higher motile spermatozoa recovery). In a fourth experiment, we tried three modifications of the freezing protocol, for improving the freezability of low concentration samples: prior removal of seminal plasma; replacing extender (second fraction) for pure glycerol to reduce dilution; and performing only the dilution, not the second dilution. No differences were found, although only three samples could be used. Both Triladyl® and Andromed® were deemed appropriate for freezing Iberian red deer semen, and the rich fraction should be selected for freezing.
xmlui.dri2xhtml.METS-1.0.item-desfisica: P. 628-638
xmlui.dri2xhtml.METS-1.0.item-peerreviewed: SI
Publisher: Elsevier
xmlui.dri2xhtml.METS-1.0.item-citation: Theriogenology, 2009, vol. 71, n. 4
URI: http://hdl.handle.net/10612/10288
Date: 2009-03-01
xmlui.dri2xhtml.METS-1.0.item-tipo: info:eu-repo/semantics/article
Subject: Veterinaria
xmlui.dri2xhtml.METS-1.0.item-palclave: Sperm cryopreservation
Electroejaculation
Extenders
Red deer
Semen fractions
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