Quality, oxidative markers and DNA damage (DNA) fragmentation of red deer thawed spermatozoa after incubation at 37 °C in presence of several antioxidants

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Quality, oxidative markers and DNA damage (DNA) fragmentation of red deer thawed spermatozoa after incubation at 37 °C in presence of several antioxidants

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dc.contributor Facultad de Ciencias Biologicas y Ambientales es_ES
dc.contributor.author Mata Campuzano, María
dc.contributor.author Álvarez Rodríguez, Manuel
dc.contributor.author Olmo de Medina, Enrique del
dc.contributor.author Fernández Santos, María Rocío
dc.contributor.author Garde López-Brea, Julián
dc.contributor.author Martínez Pastor, Felipe
dc.contributor.other Biologia Celular es_ES
dc.date 2012-09-15
dc.date.accessioned 2019-05-07T09:34:07Z
dc.date.available 2019-05-07T09:34:07Z
dc.date.issued 2019-05-07
dc.identifier.citation Theriogenology, 2012 vol. 78, n. 5 es_ES
dc.identifier.other https://www.sciencedirect.com/science/article/pii/S0093691X11006686#! es_ES
dc.identifier.uri http://hdl.handle.net/10612/10670
dc.description P. 1005-1019 es_ES
dc.description.abstract Antioxidants may be useful for supplementing sperm extenders. We have tested dehydroascorbic acid (DHA), TEMPOL, N-acetyl-cysteine (NAC) and rutin on epididymal spermatozoa from red deer, during incubation at 37 °C. Cryopreserved spermatozoa were thawed, washed and incubated with 1 mm or 0.1 mm of each antioxidant, including oxidative stress (Fe2+/ascorbate). Motility (CASA and clustering of subpopulations), viability, mitochondrial membrane potential, and acrosomal status were assessed at 2 and 4 h. Lipoperoxidation, intracellular reactive oxygen species (ROS) and DNA damage (DNA) status (TUNEL) were checked at 4 h. Oxidative stress increased ROS, lipoperoxidation and DNA damage. Overall, antioxidants negatively affected motility and physiological parameters. Only DHA 1 mm protected motility, increasing the fast and progressive subpopulation. However, it had a detrimental effect on acrosomal and DNA status, in absence of oxidative stress. Tempol and rutin efficiently reduced lipoperoxidation, ROS, and DNA damage in presence of oxidative stress. NAC was not as efficient as TEMPOL or rutin reducing lipoperoxidation or protecting DNA, and did not reduce ROS, but its negative effects were lower than the other antioxidants when used at 1 mm, increasing the subpopulation of hyperactivated-like spermatozoa at 2 h. Our results show that these antioxidants have mixed effects when spermatozoa are incubated at physiological temperatures. DHA may not be suitable because of prooxidant effects, but TEMPOL, NAC and rutin may be considered for cryopreservation trials. In general, exposure of red deer spermatozoa to these antioxidants should be limited to low temperatures, when only protective effects may develop. es_ES
dc.language eng es_ES
dc.publisher Elsevier es_ES
dc.subject Veterinaria es_ES
dc.subject.other Red deer es_ES
dc.subject.other Spermatozoa es_ES
dc.subject.other Antioxidant es_ES
dc.subject.other Oxidative stress es_ES
dc.subject.other DNA damage es_ES
dc.title Quality, oxidative markers and DNA damage (DNA) fragmentation of red deer thawed spermatozoa after incubation at 37 °C in presence of several antioxidants es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.description.peerreviewed SI es_ES

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