Evaluation of two antimicrobial packaging films against Escherichia coli O157:H7 strains in vitro and during storage of a spanish ripened sheep cheese (zamorano)

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Evaluation of two antimicrobial packaging films against Escherichia coli O157:H7 strains in vitro and during storage of a spanish ripened sheep cheese (zamorano)

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dc.contributor Facultad de Veterinaria es_ES
dc.contributor.author Otero, Verónica
dc.contributor.author Becerril González, Raquel
dc.contributor.author Santos, Jesús
dc.contributor.author Rodríguez Calleja, José María
dc.contributor.author Nerín, Cristina
dc.contributor.author García López, María Luisa
dc.contributor.other Nutricion y Bromatologia es_ES
dc.date 2014-02-23
dc.date.accessioned 2015-02-25T10:17:48Z
dc.date.available 2015-02-25T10:17:48Z
dc.date.issued 2015-02-25
dc.identifier.citation Food Control, 42 es_ES
dc.identifier.uri http://hdl.handle.net/10612/4166
dc.description P. 296-302 es_ES
dc.description.abstract Entidades financiadoras: Junta de Castilla y León (Ayuda Grupo de Excelencia GR155 y Proyecto de Investigación LE331A12-2). Proyecto de Investigación AGL2011-26118/ALI. The antimicrobial activity of two packaging films (polypropylene –PP- and polyethylene terephthalate –PET-) coated with different concentrations (0, 4, 6 and 8%) of essential oil from Origanum vulgare (OR) and Ethyl Lauroyl Arginate HCl (LAE) was tested against two E. coli O157:H7 strains using in vitro systems and a raw milk sheep cheese model (Zamorano). The influence of the antimicrobials on the sensory attributes of cheese was also evaluated. For both strains, the MBC (minimum bactericidal concentration) values were identical to their respective MIC (minimum inhibitory concentration) values and lower for LAE (25 mg/l) than for OR (200-400 mg/l). PP and PET films coated with OR were tested by a vapour phase assay and the Japanish Standard method (JIS Z 2801:2000). Both films coated with LAE were tested by an agar diffusion method. Overall, in vitro tests were effective against both strains. The inhibitory activity depended on the active compound concentration, the target strain and the packaging material, PET being more effective than PP. For inoculated cheese slices, OR and LAE PP films did not effectively decrease E. coli O157:H7 counts after 7-days cold storage. PET films incorporating 6 and 8 % of OR and LAE significantly (p< 0.05) decreased the numbers of both strains and also did 4% for the reference and wild strain depending on the antimicrobial. LAE PP, OR PET and LAE PET did not significantly (p> 0.05) affect sensorial characteristics of Zamorano cheese. Packaging with PET films coated with  6% LAE concentrations might be useful in reducing E. coli O157:H7 numbers in sheep cheese es_ES
dc.language eng es_ES
dc.publisher Elsevier es_ES
dc.subject Tecnología de los alimentos es_ES
dc.subject Veterinaria es_ES
dc.subject.other E. coli O157:H7 es_ES
dc.subject.other Ethyl Lauroyl Arginate es_ES
dc.subject.other LAE es_ES
dc.subject.other Antimicrobial es_ES
dc.subject.other Queso de oveja es_ES
dc.subject.other Essential oils es_ES
dc.subject.other Zamora es_ES
dc.subject.other Active packaging es_ES
dc.title Evaluation of two antimicrobial packaging films against Escherichia coli O157:H7 strains in vitro and during storage of a spanish ripened sheep cheese (zamorano) es_ES
dc.type info:eu-repo/semantics/article es_ES
dc.rights.accessRights info:eu-repo/semantics/openAccess es_ES

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